Infertility treatment

Additional methods of treatment

Micromanipulation techniques of egg fertilization by sperm

ICSI – Intracytoplasmic Sperm Injection

Intracytoplasmic Sperm Injection (ICSI) is an egg fertilization procedure using direct injection of a sperm inside a mature egg. The sperm is selected on the basis of its morphology and motility. Alternatively, one of the sperm selection methods may be added before performing the ICSI itself.

 We recommend ICSI:

  • in case of disorders detected by semen analysis,
  • in idiopathic infertility,
  • in female patients over the age of 37,
  • in endometriosis
  • if the previous IVF did not result in adequate fertilization of the eggs during standard fertilization.

The price of the procedure (1 – 3 oocytes) €250. This procedure is not reimbursed by the health insurance company. Individuals insured in Union health insurance company who meet the indication criteria will pay €50.

The price of the procedure (4 oocytes and more) €400. This procedure is not reimbursed by the health insurance company. Individuals insured in Union health insurance company who meet the indication criteria will pay €200.

IMSI – Intracytoplasmic Morphologically Selected Sperm Injection

The method of sperm selection in ICSI, which is based on the maximum microscopic magnification of a single sperm. This is a modification of the ICSI method, in which the embryologist can assess even the smallest sperm morphology defects and thus select the sperm with optimal morphology for fertilizing the egg. The IMSI method cannot be applied in case of a very low sperm count in the ejaculate.


The price of the procedure €150. This procedure is not reimbursed by the health insurance company.

PICSI – Pre-selected Intracytoplasmic Sperm Injection

Sperm selection method for ICSI, which is based on the assessment of irregularities of the sperm surface membrane. Prior to ICSI itself, sperm is overlaid over a microplate that resembles zona pellucida (the egg coat) with its composition. Subsequently, only the sperm that is firmly attached to the plate is used to fertilize the eggs. It is assumed that the sperm thus obtained is genetically healthier and should stand the highest chance of fertilizing the egg. 

When should this method be used?

  • In patients with pathological semen analysis parameters
  • In suboptimal embryonic development in previous IVF cycles, if we assume a problem associated with the sperm
  • In repeated unsuccessful IVF cycles with embryo transfer of quality embryos.

The price of the procedure €120. This procedure is not reimbursed by the health insurance company.

Oosight spindle view

A special microscopic technique that enables the imaging of the mitotic spindle of the egg, thus allowing the correct timing of egg fertilization using the ICSI method. We recommend this procedure in patients over the age of 37, in women with low egg yield, in patients who had inadequate embryo development in the previous IVF cycle despite normal semen analysis parameters (of their partner), in eggs that do not have an optimal microscopic image, when using cryopreserved eggs and in low percentage of fertilized eggs in the previous IVF-ICSI cycle. 

The price of the procedure €300. This procedure is not reimbursed by the health insurance company.


Sperm separation techniques

MACS – Magnetically Activated Sperm Selection

A method of sperm selection that is able to separate the damaged (apoptotic) sperms with a high percentage of fragmentation of genetic information from other sperms. The result is the separation of sperms with good genetic make-up (undamaged DNA), which stand the highest chance of fertilizing the egg. Unlike IMSI and PICSI, sperms separated under this protocol can be used for all methods of assisted reproduction – insemination, IVF, ICSI and it is even possible to cryopreserve the obtained sperms for further use in the future. 

The price of the procedure is €270. This procedure is not reimbursed by the health insurance company.

MSS (Microfluidic sperm sorting)

Sperm selection method based on changes in sperm motility depending on their quality. The separation is performed on a special plate with microchannels, which function as a filter and select the sperm with optimal motility and good fertilization abilities. Unlike IMSI and PICSI, sperm separated under this protocol can be used for all methods of assisted reproduction – insemination, IVF, ICSI and it is even possible to cryopreserve the obtained sperm for further use in the future.

The price of the procedure €250. This procedure is not reimbursed by the health insurance company.


Embryo culture methods:

Prolonged embryo culture (until day 5 of embryo development)

Embryo culture in laboratory conditions up to the stage of expanded blastocyst, 5-6 days after fertilization. The success of blastocyst transfer is higher compared to transfers of embryos in lower developmental stages, because in a large number of embryos, the division stops between the day 3 and 4 of their development. The transfer of blastocysts prevents the transfer of those embryos which would stop developing in this period under laboratory conditions. The suitability of prolonged embryo culture is assessed by the number of fertilized oocytes and also depends on a multitude of other factors and should be discussed with the embryologist.

Price of the procedure €200. This procedure is not reimbursed by the health insurance company.

Embryo culture using Timelapse System

Special embryo culturing device based on the principle of individual culture (each embryo is cultured in a special dedicated microwell). The device takes a photo of each embryo every 20-30 minutes and then processes these photos into a video loop. The advantage of such culturing procedure is that not only it is unnecessary to take the embryo out from the incubator during the whole culture process (resulting in constant conditions for the embryo) but also the embryologist has the possibility to assess the regularity and time sequence of cell division of each embryo and then select the embryo with the highest implant potential for transfer.

The price of the procedure €300. This procedure is not reimbursed by the health insurance company.

Embryo culture in a multi-chamber incubator

When culturing embryos in a multi-chamber incubator, each patient has a tiny incubator chamber dedicated to her own embryos only. In contrast to conventional culture, it is not necessary to disturb the optimal atmosphere, which is essential for the correct cell division of embryos whenever the embryologist needs to check the embryos of a different patient. The permanent atmosphere in the incubator thus improves the early embryonic development thus improving the chances of embryo implantation in the uterus and its further development. 
The price of the procedure €100. This procedure is not reimbursed by the health insurance company.

EmbryoGen – culture medium

A special culture medium for patients who have suffered recurrent miscarriages (pregnancy losses) in the past. It contains special growth factors (GM-CSM) that increase the percentage of embryos with optimal cell division. In addition to patients with recurrent pregnancy losses, it is also recommended in patients who suffered from repeated failure of embryo implantation following the transfer as well as to patients with idiopathic infertility. Embryos can be cultured in the medium 3 days after fertilization. 
The price of the procedure €100. This procedure is not reimbursed by the health insurance company.

EmbryoGen/BlastGen

A special culture medium for patients who have suffered recurrent miscarriages (pregnancy losses) in the past. It contains special growth factors (GM-CSM) that increase the percentage of embryos with optimal cell division. In addition to patients with recurrent pregnancy losses, it is also recommended in patients who suffered from repeated failure of embryo implantation following the transfer as well as to patients with idiopathic infertility. Embryos can be cultured in the medium for 6 days after fertilization. 
The price of the procedure €120. This procedure is not reimbursed by the health insurance company.


Additional methods for embryo transfer:

Laser Assisted Hatching (AH)

Disruption of the embryo shell with a laser beam. Up to the day 6 following fertilization, the embryo is protected by a special coat – zona pellucida. It needs to leave this coat to be able to implant itself in the uterus. An envelope that is too strong may be the reason why the embryo cannot leave the shell and is therefore not able to implant itself in the uterus. Cutting the zona pellucida with a laser beam is safe and poses no risk to further embryonic development. 

The price of the procedure €150. This procedure is not reimbursed by the health insurance company.

Laser-Assisted Zona Thinning (LAZT)

Disruption of the embryo shell with a laser beam. Up to the day 6 following fertilization, the embryo is protected by a special coat – zona pellucida. It needs to leave this coat to be able to implant itself in the uterus. An envelope that is too strong may be the reason why the embryo cannot leave the shell and is therefore not able to implant itself in the uterus. Thinning of the zona pellucida on a certain area with a laser beam is safe and poses no risk of endangering the further development of the embryo. 

The price of the procedure €170. This procedure is not reimbursed by the health insurance company.

Embryo glue

“Embryo glue” is a lay term used for a special medium (solution) in which the embryo is transferred to the uterus. The chemical composition of this medium is very similar to that of the fluid normally found in the uterus and has a higher density compared to conventional embryo transfer media. This should ensure both optimal conditions for further development and cell division of the embryo, as well as minimize the risk of undesired embryo moves within the female genital tract after embryo transfer. 

The price of the procedure €120. This procedure is not reimbursed by the health insurance company.

  • VŠZP
  • UNION
  • Dôvera
  • Self-payers
TitleThe amount to be paid
Oocyte fertilization techniques
Intracytoplasmic Sperm Injection (ICSI) 1 - 3 oocytesThe amount to be paid
250 €250 €50 €250 €
Oocytes (ICSI) during the 3rd cycle completed in our centerThe amount to be paid
0 €0 €0 €0 €
Intracytoplasmic Sperm Injection (ICSI) of 4 or more oocytesThe amount to be paid
400 €400 €200 €400 €
Intracytoplasmic Injection of Morphologically-Selected sperm (IMSI) The amount to be paid
150 €150 €150 €150 €
Fertilization of eggs using the ICSI method with the Oosight system - spindle view The amount to be paid
300 €300 €300 €300 €
Sperm selection method
Intracytoplazmatická injekcia preselektovanej spermie (PICSI)The amount to be paid
120 €120 €120 €120 €
Magneticky aktivovaná selekcia spermií (MACS)The amount to be paid
270 €270 €270 €270 €
Microfluidic sperm sorting (MSS)The amount to be paid
250 €250 €250 €250 €
Methods of embryo culturing
Prolonged culture (embryo culture until day 5 of embryo developmentThe amount to be paid
200 €200 €200 €200 €
Prolonged culturing (embryo culture until day 5 of its development) during the 3rd cycle completed in our centreThe amount to be paid
0 €0 €0 €0 €
Timelapse system for continuous embryo monitoringThe amount to be paid
300 €300 €300 €300 €
Timelapse system for continuous embryo monitoring during the third cycle completed in our facilityThe amount to be paid
0 €0 €0 €0 €
Embryogen (embryo culture 3 days)The amount to be paid
100 €100 €100 €100 €
Embryogen/Blastgen (embryo culture for 5 days)The amount to be paid
120 €120 €120 €120 €
Individual cultivation of embryos in a chamber incubatorThe amount to be paid
100 €100 €100 €100 €
In vitro oocyte maturation The amount to be paid
500 €500 €500 €500 €
Additional methods in embryo transfer
Laser Assisted Hatching (AH)The amount to be paid
150 €150 €150 €150 €
Laser-Assisted Zona Thinning (LAZT)The amount to be paid
170 €170 €170 €170 €
Embryo glueThe amount to be paid
120 €120 €120 €120 €

We help couples fulfill their dream of having a baby. 20 years of experience in assisted reproduction.

Book a consultation appointment and we will help you as well.

BOOK A CONSULTATION APPOINTMENT +421 800 300 000 +421 800 300 000 BOOK A CONSULTATION APPOINTMENT
Gyncare logo

Je nám ľúto, web gyncare.sk nie je možné prezerať cez váš internetový prehliadač.

Pravdepodobne používate už nepodporovanú verziu. Z tohto dôvodu vám odporúčame nainštalovať si najnovšiu verziu Microsoft Edge, Google Chrome alebo Firefox. Prostredníctvom týchto prehliadačov si užijete náš web v celej kráse.

stiahnuť prehliadač google chrome